Chapter Twenty Two:  Coordination Chemistry and Nutritonal Deficiency

 

 

Chemicals:

 

1.         Standard zinc solution (10.0 mg

            L-1 Zn2+)

            0.0440 grams ZnSO4 • 7H2O per liter, volumetric

 

2.         Zincon dye (100mg L-1)

            0{2[a(2-hydroxy-5-sulfophenylazo)benzilidene]-hydrazino}

            benzoic acid, sodium salt (80%)

 

            0.125 grams zincon per liter pH 9 buffer, volumetric

            Use the H3BO3/KCl/NaOH pH 9 buffer from Chapter 18

 

3.         Zinc unknown

Can use any dilution of #1 that the students have in their calibration (A.3), eg., 9H2O: 1 Zn2+,

            8H2O:   2Zn2+, etc.

 

4.         pH 9 buffer

            H3BO3/KCl/NaOH buffer (Chapter 18)

 

5.         Zinc tablet unknown

            See Secton B. 6 of Chemtrek

 

6.         Section C. 1

            A choice of solutions:

            Tea

            Juice from Tofu

            Apple juice

            Phytic acid, dodecasodium salt

 0.02 g per 100 mL

            Lemon juice

 

7.         Solid foods:

            Tofu, soybeans, peas, corn

Other choices could include:  fruits, spinach, grapes, other legumes, coffee beans, etc.

 

 

Supplies:

 

1.         Cotton swabs

2.         Super jumbo straws

 

3.         Slim straws

 

4.         Thin-stem pipets

 

5.         Labeled, filled microburets of each of the solutions from 6 above.  Set 1  tray each at reagent central.

 

6.         Multimeters

 

7.         CdS photocell

 

8.         Filter paper circles

            Whatman #41

 

 

Per Student:

 

1 -        Large drop microburet

 

1          SepCapTM  cap with hole

 

1 -        Slim straw

 

1 -        Microstirrer

 

1 -        Microburet

 

2 -        Wire leads with alligator clips

 

1 -        Wash bottle

 

 

From Student Kit:

 

3 -        1 x 12 well microstrips

 

1 -        24-well tray

 

1 -        96-well tray, round bottom

 

Scissors

 

Office punch

 

Lab top

 

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